Question 1
Which model of DNA replication was supported by the experiments of Meselson and Stahl?
A. Conservative
B. Semi-conservative
C. Dispersive
D. Replicative
Question 2
What is the role of DNA helicase during DNA replication?
A. Adding nucleotides to the growing strand
B. Forming phosphodiester bonds
C. Unwinding the double helix
D. Synthesizing RNA primers
Question 3
Which enzyme is responsible for adding nucleotides to the growing DNA strand during replication?
A. DNA ligase
B. DNA polymerase
C. Primase
D. Topoisomerase
Question 4
During DNA replication, which strand is synthesised continuously?
A. Lagging strand
B. Leading strand
C. Both strands are synthesised continuously
D. Neither strand is synthesised continuously
Question 5
What is the function of primase in DNA replication?
A. Sealing nicks in the sugar-phosphate backbone
B. Synthesizing RNA primers
C. Removing RNA primers
D. Relieving supercoiling of the DNA
Question 6
a) Explain the semiconservative model of DNA replication as proposed by Meselson and Stahl. How does this model ensure the accuracy of replication? [4]
b) Discuss the role and significance of DNA helicase and DNA polymerase in the process of DNA replication. [4]
Question 7
a) Describe the initiation process of DNA replication, highlighting the role of the origins of replication. [3]
b) Explain the difference between the synthesis of the leading and lagging strands during the elongation phase of DNA replication. [4]
Question 8
a) Discuss the significance of enzymes like primase and ligase in DNA replication. [3]
b) Explain how the termination of DNA replication occurs and why it is important for maintaining genomic integrity. [4]
Question 9
a) Elaborate on the semiconservative nature of DNA replication, explaining how Meselson and Stahl demonstrated this model. [4]
b) Discuss the role of DNA helicase and primase during the initiation of DNA replication. [3]
c) How does the synthesis of the lagging strand differ from that of the leading strand, and why is DNA ligase important in this process? [4]
Question 10
a) Describe the initiation of DNA replication, focusing on the role of the origins of replication and the enzymes involved. [4]
b) Explain the significance of the leading and lagging strands in the elongation phase of DNA replication. [3]
c) How does the termination phase of DNA replication contribute to the maintenance of genomic integrity? [4]