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The test for phosphate ions in a solution involves adding ammonium molybdate solution under acidic conditions.
In a more detailed explanation, the test for phosphate ions (PO4^3-) in a solution is carried out using ammonium molybdate solution. This is done under acidic conditions, typically created by adding nitric acid to the solution. The process is often referred to as the 'ammonium molybdate test' or the 'molybdenum blue test'.
Here's how it works: First, you add a few drops of nitric acid to the solution to make it acidic. Then, you add a few drops of ammonium molybdate solution. If phosphate ions are present, a yellow precipitate will form. This precipitate is ammonium phosphomolybdate.
To confirm the presence of phosphate ions, the solution is then heated gently. If phosphate ions are indeed present, the yellow precipitate will turn a distinctive blue colour. This blue colour is due to the formation of a complex ion involving molybdenum, known as 'molybdenum blue'.
It's important to note that this test is not 100% definitive. Other ions can also produce a yellow precipitate with ammonium molybdate under acidic conditions. However, the subsequent formation of the blue colour upon heating is a strong indication of the presence of phosphate ions.
Remember to always handle chemicals with care, and to conduct this test in a well-ventilated area or under a fume hood. Safety goggles and gloves should be worn to protect your eyes and skin from potential chemical splashes.
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